The spatial biology of transcription and translation in rapidly growing Escherichia coli
Single-molecule fluorescence provides high resolution spatial distributions of ribosomes and RNA polymerase (RNAP) in live, rapidly growing E.coli.Ribosomes are more strongly segregated from the nucleoids (chromosomal DNA) m3 grease gun replica than previous widefield fluorescence studies suggested.While most transcription may be co-translational,